SCIEX TripleQuad 7500 wiff2 import error

support
SCIEX TripleQuad 7500 wiff2 import error roosso  2021-04-19
 

Hi,

We have recently bought a Sciex Triple Quad 7500, which only generates .wiff2 files. However, I am unable to import these files into Skyline. Whenever I try to do so, I get the following error message:

At 22:35:
Failed importing results file 'Filename'.
[Experiment2Impl::ctor()] Object reference not set to an instance of an object.
pwiz.Skyline.Model.Results.ChromCacheBuildException: Failed importing results file 'Filename'.
[Experiment2Impl::ctor()] Object reference not set to an instance of an object. ---> System.Exception: [Experiment2Impl::ctor()] Object reference not set to an instance of an object.
at pwiz.CLI.msdata.ReaderList.read(String filename, MSData result, Int32 runIndex, ReaderConfig config)
at pwiz.ProteowizardWrapper.MsDataFileImpl..ctor(String path, Int32 sampleIndex, LockMassParameters lockmassParameters, Boolean simAsSpectra, Boolean srmAsSpectra, Boolean acceptZeroLengthSpectra, Boolean requireVendorCentroidedMS1, Boolean requireVendorCentroidedMS2, Boolean ignoreZeroIntensityPoints, Int32 preferOnlyMsLevel, Boolean combineIonMobilitySpectra, Boolean trimNativeId) in C:\proj\skyline_20_2_x64\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 194
at pwiz.Skyline.Model.Results.MsDataFilePath.OpenMsDataFile(Boolean simAsSpectra, Boolean preferOnlyMs1, Boolean centroidMs1, Boolean centroidMs2, Boolean ignoreZeroIntensityPoints) in C:\proj\skyline_20_2_x64\pwiz_tools\Skyline\Model\Results\MsDataFilePath.cs:line 291
at pwiz.Skyline.Model.Results.ChromCacheBuilder.BuildCache() in C:\proj\skyline_20_2_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 188
--- End of inner exception stack trace ---

Is there any way I would be able to solve this issue by changing a specific setting, or is Skyline not (yet) compatible with SCIEX Triple Quad 7500 wiff2 data? I am able to open the files using Sciex OS, so I am 100% sure the files are not corrupt.

Thanks for the help!