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Spectral Library generation using DIA raw workflow crashes

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Spectral Library generation using DIA raw workflow crashes alai  2021-04-18 02:47
 

Hi all,

I am following the DIA raw workflow using Skyline daily on some files collected using a TOF instrument. However, DIA umpire hangs at step 7/7 (widow something?) by using all the available memory (32 Gb).

I have previously tried using the same workflow on a sample set (ecoli/yeast/human) and it worked fine.

Thanks
 
 
Matt Chambers responded:  2021-04-19 08:28

Hi,

Did you override any of the "Additional Settings"? If so, which ones and to what values? If not, the easiest way for us to reproduce this would be to run on your dataset. Are you able to share it?

Thanks,
-Matt