Hi Team, during Kendra's tutorial on isotope tracing today there was a discussion about, and a request for, Skyline to handle fine isotope structure for non-monoisotopic ions, above an instrument resolving power which the masses of 15N or 13C isotopes would be different (for instance). Currently, in order to calculate isotope incorporation for amino acids, Kendra is calculating A+1 and A+2 outside of Skyline because for most instances there are more than one ion in the fine stucture when the resolution is high enough. An example of a Skyline document with some data included is attached.

Cheers

W