Brendan MacLean responded: |
2013-07-24 09:35 |
Hi Richard,
Skyline should be able to build a spectral library directly from a Mascot .dat file or a Proteome Discoverer .msf file. Does either of those allow you to achieve what you are looking to do?
Thanks for your interest in Skyline.
--Brendan |
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r g beniston responded: |
2013-07-25 01:38 |
Hi Brendan,
Thanks for the quick reply. That solution sounds ideal, but under peptide settings-library tab-build library and add files I can't select the .msf file as it's not an option. If I select an .msf file through looking for any files it state an error of not being a valid library input file. AM I missing something or doing something wrong?
Best wishes.
Richard |
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Brendan MacLean responded: |
2013-07-30 07:01 |
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whwind83 responded: |
2014-10-30 19:49 |
To whom it may concern:
I am new to Skyline and have just installed Skyline (64-bit) 2.6.0.6851.
I am trying to build a spectral library by importing the Proteome Discoverer 1.4 database search results .msf file into Skyline. I am using the function icon of Import DDA Peptide Search. I can add the .msf file, and the system undergoes parsing spectra, reading spectra, and filtering redundant library. However, it ends up with an empty screen showing that "spectrum information unavailable".
Did I miss anything?
Many Thanks and Warm Regards,
Wang Han |
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Kaipo Tamura responded: |
2014-10-31 10:04 |
Hi Wang Han,
I have contacted you through email about this issue.
Thanks,
Kaipo |
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poorichaya s responded: |
2024-02-01 17:21 |
I am a new user for skyline
I also found a problem in this issue too.
How can i fix it?
Thanks,
Poorichaya |
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Nick Shulman responded: |
2024-02-02 07:22 |
Poorichaya,
Can you give us more information about what you are trying to do and what is not working?
Are you seeing some sort of error message?
If you send us some files we can try to figure out what is going on.
Files which are less than 50MB can be attached to this support request.
You can upload larger files here:
https://skyline.ms/files.url
-- Nick |
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