|Lipodomics: How to apply single calibration curve (done with one representative molecule) to all members of a lipide class??||StefanW||2021-02-15|
Because of the diversity of lipids they are normally separated in group, which share a common structural motive, like head groups etc. Typically these groups shares a common MS2 fragment.
The LipidCreator tool is very helpful in creating the corresponding transition lists.
For quantification it is usually to generate a calibration curve with one of the group member (i.e. PS (18:1, 16:0) ). Subsequently these calibration curve is used to quantify all group members. For this purpose the "slope" of PS(18:1,16:0) has to be assigned to all PS species (i.e. PS(20:0,16:0) or PS(16:1, 18:1) etc etc).
Inclusion this feature will procreate skyline to a unique and outstanding lipid tool...in my mind....
Thanks a lot for a comment on this support case.