Simple precursor ratios

Simple precursor ratios chiva cristina  2021-02-02

Dear Skyline team,
I hope you are all doing well!
I have a question regarding the last version of Skyline daily. I have noticed there is a new checkbox (or maybe it is not so new but I have just discover it) in the Quantification tab under Peptide Settings called "Simple precursor ratios". Could you tell me what does it does and when can be helpful?
Thanks a lot

Nick Shulman responded:  2021-02-02
It's useful if your light and heavy molecules have a different number of transitions, and you want Skyline to calculate ratios by simply adding up the light area and dividing by the heavy area.

The motivation for this feature was this support request:
-- Nick
Nick Shulman responded:  2021-02-02

By the way, did you notice the tooltip which appears when you hover over that checkbox?
Do you think we need to make the tooltip more descriptive? Does it need an extra sentence or something?

The tooltip currently says:
If checked, ratios between precursors will be calculated as the ratio of the sum of transition areas.
Transitions from the two precursors will not be paired with each other for the purpose of handling missing peaks or calculating ratio dot products.

-- Nick

chiva cristina responded:  2021-02-02

Hi Nick,
I think the explanation is good enough it is just that I didn't noticed it was there.