When you see the "No product ion chromatograms found" it means that there are some chromatograms, but they are MS1 chromatograms, and you have told Skyline to only show you MS2 chromatograms.

You should right-click on the chromatogram graph and choose "Transitions > All". I believe you have selected the option "Transitions > Products".

It is also possible that something else is going wrong. If "Transitions > All" does not fix it, you can send us your Skyline document.

In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms.

If that .zip file is less than 50MB you can attach it to this support request. Otherwise you can upload it here:
https://skyline.ms/files.url

-- Nick

Nick,

Thank you so much for your prompt reply. I tried that and still nothing. Here is the file attached.

HHB!

The product ions that you have chromatograms for are the singly charged y6+, y7+ and y8+ ions, but those fragment ions do not appear in your Targets tree.

The reason that Skyline did not add those ions to your Targets tree is that "1" is not included in the Ion Charges list at "Settings > Transition Settings > Filter".

You can add those fragment ions to your Targets tree by right-clicking on the precursor "886.9827++" and choosing "Pick Children".
You will have to click on the filter button on the child picker window in order for Skyline to show you the fragment ions which are being filtered out.
After you have unselected the filter button, you will see that there is a green dot next to y6, y7 and y8, so you will know that those are the ions you should add.

Another way to get those fragment ions into your document would be to go to:
Settings > Transition Settings > Filter
and make it so that "1" is contained in the Ion Charges list.
-- Nick

Nick,

That's great, no wonder I couldn't see it. Thank you for all the help!!!!

HHB!