|Different results from raw data that are nearly the same in MassLynx||anna walke||2021-01-07|
Dear SkyLine Team,
I'm working with a simple method on a waters Premier Pro QToF to weekly check system performance by analyzing a standard of angiotensin II. Measurement is performed by Full Scan (function 1) and MS/MS scan (function 2) in parallel over the entire time range of the method.
Unfortunately when analyzing my raw data with Skyline (version 184.108.40.2063) I get very different results compared to manually analyzing the same data in MassLynx. I added screenshots of this and also attached the corresponding information of my LC-MS experiment. Within the experiment report I highlighted the different paramters in yellow, but there are only little differences.
I do not understand why the same SkyLine Document (also attached below) with same molecule and transition settings gives that different results after integration.
Could you please help me or give a hint towards a possible solution?
With kind regards,