Peptide Atlas Download for human library build

Peptide Atlas Download for human library build daraghob  2020-12-16

I am attempting to generate a new spectral library to achieve better target peptide coverage for a transition list I am generating for a future MRM experiment.

My issue is trying to discern which file to download on the peptide atlas builds page (
There are many options for the human build but I can't seem to find the appropriate data format (.pep.xml) for the library build.

Any help with this would be greatly appreciated.
Many thanks,
Nick Shulman responded:  2020-12-16

Skyline can use libraries in the ".sptxt" format directly without going through the process of building a BilbioSpec spectral library (.blib).

To use a .sptxt library, you go to:
Settings > Peptide Settings > Edit List > Add

-- Nick