It looks like Skyline was not able to find any MS2 spectra that matched the 487.2213 precursor m/z of your heavy peptide.
You should look at your mass spec data file and make sure that there really are some MS2 spectra with that isolation window.
(I usually use a program called SeeMS.exe to look at mass spec data files. You can install SeeMS.exe along with the rest of ProteoWizard from http://proteowizard.sourceforge.net
/. You also might have your own instrument vendor software for looking at mass spec data files.)
Another thing that often happens when you are doing a Targeted acquisition in Skyline is that there are some spectra in the file which you intended to be associated with that precursor, but which Skyline is assigning to a different precursor. If the MS/MS filtering Acquisition method (specified at "Settings > Transition Settings > Full Scan") is "Targeted", then Skyline will assign any particular MS2 spectrum to whichever precursor had a m/z which was closest to what was isolated for that scan. If some of your MS2 isolation windows are intended to be used for more than one precursor, then you should change the Acquisition Method to "DIA", and set the Isolation Scheme to probably "Results Only".
If you are still stumped about what is going wrong, you can send us your Skyline document and one of your raw files.
In Skyline you can use the menu item "File > Share" to create a .zip file containing your Skyline document and supporting files including extracted chromatograms. If that .zip file and your raw file are less than 50MB, you can attach them to this support request. Otherwise, you can upload them here: