Spectral Library Creation

Spectral Library Creation pg23  2020-11-06


I would like to create a spectral libray for a targeted method set-up similar to the one that is included in the Targeted Method Edit Tutorial (from Yeast).
I have been searching for potential spectra at PRIDE, but I fail to download the right format to upload in the library (Peptide Settings). Where can I find suitable files for spectral library creation?

Thank you

Brian Pratt responded:  2020-11-06

I believe PRIDE provides libraries in .MSP format (https://www.ebi.ac.uk/pride/spectrumlibrary), which is a format Skyline should be able to add directly to its Library list.

Thanks for using the Skyline support board!

Brian Pratt