Error on Importing after installing a UV/VIS detector

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Error on Importing after installing a UV/VIS detector chris brown-2  2020-11-02
 

Hello -

I received an error message when attempting to import data to Skyline 20.1.0.31.

Up until I configured a UV/Vis detector on the instrument I had been able to import data just fine. After installing that detector, Skyline is unable to import the .raw files. If the work around is just to de-configure the UV/Vis detector, that is fine.

I am attaching the error message that is returned in the import box below.

Thanks, CJB


At 8:15 AM:
Failed importing results file 'D:\Studies\Lumos-DailyQC\Data\100ppb-HypersilGold_20201031014802.raw'.
error reading spectrum controllerType=4 controllerNumber=1 scan=1

Inner exceptions:
Exception type: System.Reflection.TargetInvocationException
Error message: error reading spectrum controllerType=4 controllerNumber=1 scan=1
error reading spectrum controllerType=4 controllerNumber=1 scan=1
at pwiz.Skyline.Util.Helpers.WrapAndThrowException(Exception x) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Util\Util.cs:line 1909
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.Spectra.NextSpectrum() in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 915
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.ExtractChromatogramsLocked() in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 258
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.ExtractChromatograms() in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 238
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.SetRequestOrder(IList`1 chromatogramRequestOrder) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 575
at pwiz.Skyline.Model.Results.ChromCacheBuilder.Read(ChromDataProvider provider) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 381
at pwiz.Skyline.Model.Results.ChromCacheBuilder.BuildCache() in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 252

Exception type: System.Exception
Error message: error reading spectrum controllerType=4 controllerNumber=1 scan=1
error reading spectrum controllerType=4 controllerNumber=1 scan=1
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.Spectra.ReadSpectrum(Int32& i) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 1125
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.Spectra.Read() in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 952
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.Spectra.<RunAsync>b__33_0() in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 809

Exception type: System.Exception
Error message: [RawFileThreadImpl::getTrailerExtraValue(SPS Masses Continued:)] GetTrailerExtraValue
[RawFileThreadImpl::getTrailerExtraValue(SPS Masses Continued:)] GetTrailerExtraValue
at pwiz.CLI.msdata.SpectrumList.spectrum(Int32 index, DetailLevel detailLevel)
at pwiz.ProteowizardWrapper.MsDataFileImpl.GetCachedSpectrum(Int32 scanIndex, DetailLevel detailLevel) in C:\proj\skyline_20_1_x64\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 1056
at pwiz.ProteowizardWrapper.MsDataFileImpl.GetMetaDataValue[TVal](Int32 scanIndex, Func2 getValue, Func2 isUsableValue, Func2 returnValue, DetailLevel& detailLevel) in C:\proj\skyline_20_1_x64\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 1116 at pwiz.ProteowizardWrapper.MsDataFileImpl.GetMetaDataValue[TVal](Int32 scanIndex, Func2 getValue, Func2 isUsableValue, Func2 returnValue, DetailLevel& detailLevel) in C:\proj\skyline_20_1_x64\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 1123
at pwiz.ProteowizardWrapper.MsDataFileImpl.GetMsLevel(Int32 scanIndex) in C:\proj\skyline_20_1_x64\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 1128
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.LookaheadContext.GetMsLevel(Int32 index) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 1359
at pwiz.Skyline.Model.Results.SpectraChromDataProvider.Spectra.ReadSpectrum(Int32& i) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 1036

 
 
matt.chambers42 responded:  2020-11-02

Hi Chris,

I'm surprised Skyline is not simply able to ignore these spectra (PDA spectra I think). Can you share the RAW file? It seems to be causing an error reading the trailer extra values.