Drif time predictor

support
Drif time predictor mariangela valletta  2020-10-30
 

Hi,
I have skyline 20.2 64 bit on my Windows 10. To get familiar with skyline and ion mobility data , l followed the tutorial 'Skyline Ion Mobility Spectrum Filtering' and I downloaded zip file. I read that with new version of skyline IMS filtering settings moved to Transition Settings - Ion Mobility. My problems is: 'Use Results' button is not clickable. Why?

Thank you

 
 
Brian Pratt responded:  2020-10-30

You'll need to use the Create button to create the library before you can Use Results to populate it.

Thanks for using the Skyline support board!

Brian Pratt

 
mariangela valletta responded:  2020-10-31
Thank you very much for the quick reply.

I create a library, but cliking on the apex of the yellow chromatogram I can't see the horizontal violet range showing the filter imposed.
For my analyses, I am using skyline for analysis of peptide library, analysed with HDMSe. I import FASTA file and then results ( raw file).
It's correct?
I am interested in understanding Ion Mobility Separation.


Thank you
 
Brian Pratt responded:  2020-10-31
You just need to reimport the raw file (Edit > Manage Results > Re-import) so that Skyline can use the newly created ion mobility library for improved chromatogram extraction.

Best,

Brian
 
mariangela valletta responded:  2020-11-02
I followed your instructions, but clicking on the apex of the y6 chromatogram to show the Full-Scan graph, the horizontal violet range showing the filter imposed by the drift time predictor is not present.
In this case it means that the filter has not been applied?
I have attached the screenshot

Thank you
 
Brian Pratt responded:  2020-11-02
Hi Mariangela,

It would probably be most efficient if I could have a look at your Skyline document (save it with File > Share > Complete, this creates a .sky.zip file that also includes the library) and the raw files that are giving you trouble. You can upload to http://skyline.ms/files.url or we can arrange something private if you wish.

Best regards,

Brian
 
mariangela valletta responded:  2020-11-02
Thank you very much for your time. I have uploaded Skyline document named Library_HDMSe_MARIANGELA.
We can arrange something private, this is my mail: mariangela.valletta@unicampania.it.

Thank you.
 
Brian Pratt responded:  2020-11-05
OK, with your files in hand I find that simply doing the reimport works just as expected.

If you find that this is not the case for you, we can investigate further, but I think you just missed a step.

Best regards,

Brian