ERROR: No Spectra were found for the new library

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ERROR: No Spectra were found for the new library lake n paul  2020-10-13
 

All,

Excited to see the DDA search implemented into Skyline. However, I tried unsuccessfully to run a search. The search goes to completion however I don't get any spectra for the detected peptides. The XIC for the peptides are present but no MS/MS spectra. An error (see attached) is given.

Help!

Lake

 
 
matt.chambers42 responded:  2020-10-14

Hi Lake,

There could be several reasons for this; the easiest way for us to diagnose is to get one of the MS files and the FASTA and try to run it ourselves. Also tell us what settings you used for the search.

Thanks,
-Matt