Where did you get stuck? Can you send us your Skyline document?
In Skyline, you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms.
If that .zip file is less than 50MB you can attach it to this support request. Otherwise, you can upload it here:
If you are having trouble getting started, then these are the steps that I would recommend:
1. Add the peptides to your Skyline document using the menu item:
Edit > Insert > Peptides
2. Tell Skyline about your heavy isotope modification by going to:
Settings > Peptide Settings > Modifications
and click the lower "Edit List" button (the one next to "Isotope Modifications") and then click "Add".
(I am not sure I understand what type of heavy label you are using, but Skyline knows about nearly all of the unimod modifications, so your modification is probably already in the list, but if it is not, you can define it yourself by telling Skyline which atoms are heavy).
3. Tell Skyline that you are interesting in extracting MS1 chromatograms by going to:
Settings > Transition Settings > Full Scan
and choose something other than "None" under "Isotope Peaks Included".
4. Tell Skyline that you are interested in precursors by going to:
Settings > Transition Settings > Filter
and make sure that "p" is included in the "Ion Types" box.
5. Tell Skyline to extract chromatograms using the menu item:
File > Import > Results
After you have extracted chromatograms, you can see the results in the peak area graph (View > Peak Areas > Replicate Comparison).
You can also see the results in the document grid, probably in a column such as "Ratio to Standard".