Array dimensions exceeded supported range error on DIA .raw file import - ELIB from EncyclopeDIA

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Array dimensions exceeded supported range error on DIA .raw file import - ELIB from EncyclopeDIA cabarnescabarnes  2020-09-05
 

Hi all,

I have been doing the gas phase fractionation workflow with EncyclopeDIA to generate an ELIB from the "Save Quant Reports" in EnclyclopeDIA. Then, I import the ELIB generated after that step into Skyline and usually can import .raw DIA files cleanly and look at all the data in Skyline. I'm working on an experiment now, however, which has a lot of transitions (238,774 transitions / 58,099 peptides / 6310 proteins) and I'm importing 36 injections. I've tried the import multiple times with this library and it fails with the below error. However, if I delete all of the peptides down to a single peptide, I can get it to import just fine. When it fails, it looks like it gets all the way to the end and then fails after the visual import status graph looks finished. Is this a memory problem? Or something else?

At 11:47 AM:
Failed importing results file 'C:\TPP\data\smORF_discovery_Experiements_BAT_scWAT_huWAT_C2C12\2020_Beige_BAT_WAT_secretome_proteome\Proteome\20200731_LUM1_CPBA_EASY04_085_30_SA_90mingrad_80B_DIA_400_1000_8mzol_15k_20IIT_4e5agc_1850_02_01.raw'.
Array dimensions exceeded supported range.

Thank you so much!!

Best,

chris

 
 
Nick Shulman responded:  2020-09-05
When you saw that error, was there a "More Info" button? If you push the More Info button, it will provide a callstack which would tell us exactly where in the Skyline source code the error occurred.

If there is a more info button, could you push it and copy all the text and send it to us.

We might be able to figure out what is going wrong from that call stack, or we might need to see your .elon file.

If that .elib file is less than 50MB you can attach it to this support request.

Otherwise you can upload it here:
https://skyline.ms/files.url

--Nick