DDA Spectral Library building from Thermo Lumos , "precursor mass analyzer" option Centroided or Orbitrap

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DDA Spectral Library building from Thermo Lumos , "precursor mass analyzer" option Centroided or Orbitrap sray  2020-08-24
 

Hello All,

I'm building a spectral library from DDA peptide search results generated on Thermo Lumos.

In "Configure MS1 Full-Scan Settings" section, under "precursor mass analyzer" , which option is better, "Centroided" or "Orbitrap" (with corresponding resolution settings) ?

Thanks

 
 
Nick Shulman responded:  2020-08-25
Title: DDA Spectral Library building from Thermo Lumos ,
We recommend "centroided".

When you choose "centroided", the chromatograms that Skyline extracts usually end up having less noise because signal from other compounds is less likely to overlap with the m/z range that Skyline is summing across in each spectrum.
-- Nick