How can I select endogenous peptides not spiked-in iRT peptides for RT nomalization in Skyline?

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How can I select endogenous peptides not spiked-in iRT peptides for RT nomalization in Skyline? andyzcq  2020-07-23
 

I have acquired diaPASEF data, but I forgot to spike iRT peptide into the samples.

So how can I select endogenous peptides for RT normalization?

CiRT peptides in the CiRT calculator are not applicable.