Top 5 quantitation

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Top 5 quantitation swg42  2020-07-15
 

I'm new to using skyline and was hoping to get some help on how best to output the top 5 intensity peptides for label-free quantitation? Any suggestions?

 
 
Nick Shulman responded:  2020-07-15
If you want to get lists of numbers out of Skyline you should use the Document Grid and Reports.

This tutorial will probably be helpful:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_custom_reports

I do not know of a straightforward way in Skyline to filter the data down to just the five most intense peptides. However, you will probably be able to get the numbers that you need using the values exported from Skyline and another tool such as Microsoft Excel.
-- Nick