TMT0 Labeled Peptide as Internal Standard for TMT10 Targets

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TMT0 Labeled Peptide as Internal Standard for TMT10 Targets drothenberg  2020-07-07
 

Instead of using a heavy labeled synthetic peptide as an internal standard, I'm interested in using a TMTzero labeled peptide to compare against TMT-10plex labeled targets. I know ratios can be calculated using the Surrogate Standard option, but is it possible to have synchronized zooming and transition matching similar to heavy-labeled peptides? This would be similar to the TOMAHAQ approach, but without MS3. Thanks.