Hi There,
I have been trying to make a spectra library of about few thousands synthetic peptides from a DDA run. I was able to import the data using "mziden" and "mzxml" files from the search and mass spectrometry data, respectively. But I have two questions here, 1) how can I export all the spectra libraries into a single file in skyline?
- How could I do the peak picking for over thousand peptides, as they are so many, it is not possible for me to open them to make sure the RT is aligned.
Do I need to make sure the alignment of the RT is correct then package them?
I can send you the skyline file if that help,
Thank you, looking forward to hearing from you.
Regards,
SRS