Metabolite ID Workflow for Oligonucleotides

Metabolite ID Workflow for Oligonucleotides Richard Lam  2020-06-17

Can Skyline perform metabolite ID for oligonucleotides? If yes, any tutorials or webinars available. Thanks!

Brian Pratt responded:  2020-06-19

Hi Richard,

Skyline is for targeted mass spec, which is to say it's assumed that you already have an idea of what's in your sample. It's not a search tool per se.

That said, you can certainly populate a transition list from a spectral library, perform chromatogram extraction, and see what matches well with your sample and what doesn't. It's not a sophisticated way to approach search but might be a useful first order filter.

Thanks for using the Skyline support board!

Brian Pratt