|Is it possible to use the Skyline program for the quantitative analysis of various modified forms of the same peptide in different samples with known relative ionization coefficient k?||akulyyasov||2020-06-12|
With best regards
This Biotin Acceptor Peptide (BAP) is a non-naturally occurring peptide and is absent in the NCBI and Uniprot database.
Biotin Acceptor Peptide (BAP) - tryptic peptide for LC-MS/MS
The relative ionization coefficient k between the biotinylated and propionylated BAP peptides was estimated as k = (HP - LP)/(LB - HB), where HP corresponds to the area of TIC of heavy propionylated, LP of light propionylated, LB of light biotinylated, and HB of heavy biotinylated BAP. K were calculated for two independent experiments, giving an average value 11.9 (Kulyyassov A, et al. PUB-MS: a mass spectrometry-based method to monitor protein-protein proximity in vivo.//J Proteome Res. 2011 Oct 7;10(10):4416-27. doi: 10.1021/pr200189p).
I also uploaded one experiment in Panorama database