LOQ CV requirement iterates from low to high concentration, but high to low concentration would be better

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LOQ CV requirement iterates from low to high concentration, but high to low concentration would be better philip remes  2020-05-28
 

Hi guys,

I'm trying to use the Calibration Curve functionality, and have set the LOQ CV requirement to 20%. I notice some strange stuff going on, where sometimes a compound will have crap CV's at low concentration, then randomly have a level with a CV less than 20% when there's just noise. This level will get picked as the LOQ, even though some higher concentrations have worse CVs. Looking at the data, it's clear that the true LOQ should be at much higher concentration. To me, this looked as though the algorithm was proceeding from low concentration to high concentration, and taking the first level that meets the CV requirement as the LOQ. I would suggest that the LOQ would be more accurate if you proceeded from high concentration to low concentration, and picked the last level that meets the CV requirement. The peptide IFYNQQNHYDGSTGK is one example of this.

Thanks
Philip

 
 
Nick Shulman responded:  2020-05-28
Yes, this problem was recently pointed out by someone else.
https://skyline.ms/announcements/home/support/thread.view?rowId=44890

I will make sure to fix this before we ship Skyline 20.2.
-- Nick