method export error

support
method export error dawn dufield  2020-05-22
 
Hi.

do you know what would cause this error. We have the analyst configuration in simulation mode and seems to be able to open the methods properly. This same skyline file can be exported on a different computer with analyst. So it feels like an analyst config issue, but seems like it should work.

any suggestions

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Skyline
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An error occurred attempting to export.
ERROR: Unable to cast object of type 'Sciex.MultiQuant.AnalystImprs.ApplicationObject' to type 'Interop.Analyst.ApplicationClass'.

Command-line: Method\AbSciex\TQ\BuildQTRAPMethod -s -m "X:\Analyst Data - BioPharma\Projects\Peptide\Acquisition Methods\Trap Pepcal 10 min.dam"
Working directory: X:\Analyst Data - BioPharma\Projects\Ext\Acquisition Methods
Standard input:
X:\Analyst Data - BioPharma\Projects\Ext\Acquisition Methods\~SK14E.tmp
X:\Analyst Data - BioPharma\Projects\Ext\Acquisition Methods\test.dam
1752.439942,1930.069112,5,sequence1.xremoved this sequence info....
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OK More Info
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System.IO.IOException: ERROR: Unable to cast object of type 'Sciex.MultiQuant.AnalystImprs.ApplicationObject' to type 'Interop.Analyst.ApplicationClass'.

Command-line: Method\AbSciex\TQ\BuildQTRAPMethod -s -m "X:\Analyst Data - BioPharma\Projects\Peptide\Acquisition Methods\Trap Pepcal 10 min.dam"
Working directory: X:\Analyst Data - BioPharma\Projects\Ext\Acquisition Methods
Standard input:
X:\Analyst Data - BioPharma\Projects\Ext\Acquisition Methods\~SK14E.tmp
X:\Analyst Data - BioPharma\Projects\Ext\Acquisition Methods\test.dam
1752.439942,1930.069112,5,sequence removed....

---> System.IO.IOException: ERROR: Unable to cast object of type 'Sciex.MultiQuant.AnalystImprs.ApplicationObject' to type 'Interop.Analyst.ApplicationClass'.

Command-line: Method\AbSciex\TQ\BuildQTRAPMethod -s -m "X:\Analyst Data - BioPharma\Projects\Peptide\Acquisition Methods\Trap Pepcal 10 min.dam"
Working directory: X:\Analyst Data - BioPharma\Projects\Ext\Acquisition Methods
Standard input:
X:\Analyst Data - BioPharma\Projects\Ext\Acquisition Methods\~SK14E.tmp
X:\Analyst Data - BioPharma\Projects\Ext\Acquisition Methods\test.dam
1752.439942,1930.069112,5,sequences removed.....

   at pwiz.Common.SystemUtil.ProcessRunner.Run(ProcessStartInfo psi, String stdin, IProgressMonitor progress, IProgressStatus& status, TextWriter writer) in C:\proj\skyline_20_1_x64\pwiz_tools\Shared\Common\SystemUtil\ProcessRunner.cs:line 62
   at pwiz.Common.SystemUtil.ProcessRunner.Run(ProcessStartInfo psi, String stdin, IProgressMonitor progress, IProgressStatus& status) in C:\proj\skyline_20_1_x64\pwiz_tools\Shared\Common\SystemUtil\ProcessRunner.cs:line 57
   at pwiz.Skyline.Util.Extensions.UtilProcess.RunProcess(ProcessStartInfo psi, String stdin, String messagePrefix, IProgressMonitor progress, IProgressStatus& status) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Util\Extensions\UtilProcess.cs:line 44
   at pwiz.Skyline.Model.MethodExporter.ExportMethod(String exeName, List`1 argv, String fileName, String templateName, Dictionary`2 dictTranLists, IProgressMonitor progressMonitor) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Export.cs:line 3906
   at pwiz.Skyline.Model.AbiMethodExporter.ExportMethod(String fileName, String templateName, IProgressMonitor progressMonitor) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Export.cs:line 2397
   at pwiz.Skyline.Model.ExportProperties.<>c__DisplayClass119_0.<ExportAbiQtrapMethod>b__0(IProgressMonitor m) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Model\Export.cs:line 523
   at pwiz.Skyline.Util.ProgressWaitBroker.PerformWork(ILongWaitBroker broker) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Util\UtilUI.cs:line 125
   at pwiz.Skyline.Controls.LongWaitDlg.RunWork(Action`1 performWork) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 246
   --- End of inner exception stack trace ---
   at pwiz.Skyline.Util.Helpers.WrapAndThrowException(Exception x) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Util\Util.cs:line 1907
   at pwiz.Skyline.Controls.LongWaitDlg.PerformWork(Control parent, Int32 delayMillis, Action`1 performWork) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 194
   at pwiz.Skyline.Controls.LongWaitDlg.PerformWork(Control parent, Int32 delayMillis, Action`1 performWork) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 131
   at pwiz.Skyline.FileUI.ExportDlgProperties.PerformLongExport(Action`1 performExport) in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\FileUI\ExportMethodDlg.cs:line 2022
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Brendan MacLean responded:  2020-05-24
That really looks like a bug in the Analyst libraries which Skyline relies on to do the method export. I have pointed our contact at SCIES to this thread. Hopefully, we will get some idea of what they think on this support thread soon.

In the meantime, it is often good to try other computers with the same files and other files on the same computer to try to understand whether it is a problem specific to particular computer and installation of Analyst or something particular to your template file or the transitions Skyline is trying to export into the method.

You can, of course, also try File > Export > Transition List to see if you can get transition lists which you can then manually import into your template method inside the Analyst user interface.

Thanks for posting this to the support board. Let us know, if you make any progress in understanding the boundaries of when this happens while we are waiting for a response from SCIEX.

--Brendan
 
Kaipo responded:  2020-05-25
Hi,

Do you have the MultiQuant method option checked? From Dave Cox:
"I think they have the checkbox to create qmethods selected. It is complaining about creating a MultiQuant method, which I don’t think they are using. If they uncheck that option, I hope the acquisition method creation will work fine."

Thanks,
Kaipo
 
Kaipo responded:  2020-05-27
Dave Cox has sent this potential fix:

"It is very likely that Dawn is using this on a computer with both Analyst and SCIEX OS installed. To enable compatibility with older LIMS connectivity software, SCIEX OS will impersonate Analyst under some scenarios.

Most likely, shutting down SCIEX OS while using Skyline for method building will fix this issue."

Would you mind seeing if that resolves the issue?

Thanks,
Kaipo