10 years later, I am encountering same problem now. Can you suggest if there is a way to overcome this. I am using Agilent 6490 and the method has first 0.5 min going to waste. The transition list is same across both time segments. It is MRM method and not dMRM. Would it help if I include few unrelated transition in first 0.5 min of the run?
I am using Skyline 4.2.
Is this a segmented method again? I don't think we have any hope of getting Agilent to support this. They will again recommend dMRM.
Or this another type of issue, which I am not understanding yet?
I would likely need screenshots and maybe files to have much hope of helping.