phase 11 study 9p scheduled MRM

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phase 11 study 9p scheduled MRM Paige Riley  2009-07-24
 
Hi Brendan,

I was trying to analyze a sample set of scheduled results for the agilent qqq and have run into a snag. Skyline seems to only be able to display the first segment of the the schedule run. In my case the frist segments is from 0-10 minutes and has no pertinent information/data. I tried to attached a representative file for you to work with but because the instrument save all the data in a file I am unsure which part to send so I will attempt to email the entire folder to you.
Thanks,

Paige
 
 
Brendan responded: 2009-08-05 14:08
Hi Paige,
Investigation of this has been moving through email. There does appear to be an issue with segmented scheduled runs on Agilent instruments, or at least this one. Agilent has recommended an upgrade to dMRM, with which Skyline has been tested. But, I am still trying to get the segmented data to work better. Thanks for the report.

--Brendan
 
 
 
Alok responded:  2019-03-14
Title: Importing Agilent QqQ data with more than one time segment
Hi Brendan,

10 years later, I am encountering same problem now. Can you suggest if there is a way to overcome this. I am using Agilent 6490 and the method has first 0.5 min going to waste. The transition list is same across both time segments. It is MRM method and not dMRM. Would it help if I include few unrelated transition in first 0.5 min of the run?

I am using Skyline 4.2.

Thanks,
Alok
 
Brendan MacLean responded:  2019-03-15
Hi Paige,
Is this a segmented method again? I don't think we have any hope of getting Agilent to support this. They will again recommend dMRM.

Or this another type of issue, which I am not understanding yet?

I would likely need screenshots and maybe files to have much hope of helping.

--Brendan