Dear Skyline team,
I ran a DIA MSX experiment on QExactive HF-X set up using instructions by 5 m/z window, DIA, MSX 4.Data was collected in the profile mode. The method was set according to the template. I want do the demultiplex of RAW files by the Mscovert software, but this software displayed some error information. as shown:
Opening file "J:\lxy\LXY-data\DIA\MSX\20191218\HFX_LXY_IgG_DIA_Methods_1_MSX_dia_4_5Da_20191218_F1_R1_20191219191652.raw" for read...
Calculating SHA1 checksum...
Failed - System.Exception: SpectrumToIndices() Number of demultiplexing windows changed. Minimum window size or window boundary tolerance may be set too low.
在 pwiz.CLI.msdata.MSDataFile.write(MSData msd, String filename, WriteConfig config, IterationListenerRegistry iterationListenerRegistry)
在 MSConvertGUI.MainLogic.processFile(String filename, Config config, ReaderList readers, Map
2 usedOutputFilenames) 在 MSConvertGUI.MainLogic.Go(Config config, Map2 usedOutputFilenames)
I want to know if there is a problem with my method settings?
Or can the data produced by the HF-X not be processed by this software?
My computer system is Window 7 service pack 1, 64 bit. The skyline software version is 64 bit 126.96.36.19917. The MScovert Version is 3.0.19161-f1b0b59b1.
The raw data and skyline files are already upload , "HFX_LXY_IgG_DIA_Methods_1_MSX_dia_4_5Da_20191218_F1_R1_20191219191652.rar"
And the problem and the setting is shown in the attachments.