Error reading iRT standards transition list

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Error reading iRT standards transition list HENG  2020-03-27
 
Hello. I am learning your Skyline Importing Assay Libraries documents. And when I proceed to the following steps (see my attachments) and click OK button. The skyline showed this Error reading

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Skyline
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Error reading iRT standards transition list: Product m/z value 171.11 in peptide TPVISGGPYEYR has no matching product ion.
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OK More Info
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System.IO.InvalidDataException: Product m/z value 171.11 in peptide TPVISGGPYEYR has no matching product ion.
   at pwiz.Skyline.FileUI.CreateIrtCalculatorDlg.OkDialog() in C:\proj\skyline_20_1_x64\pwiz_tools\Skyline\FileUI\CreateIrtCalculatorDlg.cs:line 194
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I am sure I was following every steps of the document. So how can I solve this problem?

Thanks.

Best,
Heng
 
 
Nick Shulman responded:  2020-03-27
Heng,

It sounds like you are going through the steps in this tutorial:
https://skyline.ms/_webdav/home/software/Skyline/%40files/tutorials/ImportingAssayLibraries-2_6.pdf

The error is happening because the .tsv file has an ion (a2+) which is not accepted by the document's Transition Settings Filter.
To work around this error, you would go to:
Settings > Transition Settings > Filter
and change things so that "a2" is an acceptable ion.
That would involve adding "a" to the list of "Ion Types", and also change "Product Ion Selection" from to something like "ion 1".

It sounds like the steps of this tutorial need to be updated. This tutorial was originally written in 2014.

Someone might be able to point you at a more up-to-date tutorial which covers the topic you need.
-- Nick
 
HENG responded:  2020-03-27
Thanks, Nick. It looks affected by my former setting.

Now the problem is solved.

Thanks

Heng