|How to caculate the Precision,Extraction recovery or Matrix effect with skyline?||kaylee xu||2020-02-11|
Dear skyline team,
Q1: Recently, I am using Skyline for small molecular quantitative analysis. At methodological validation, I don't know how to calculated the Precision, Extraction recovery or Matrix effect with Skyline? Can you give me some steps?
Q2: If I build 10 compounds' calibrations at a time with different concentrations, eg. Compound-1( 1, 2, 5, 10, 20, 50, 100ng/mL),compound-2(2,4,10,20,40,100,200ng/mL) et.al. How to enter the concentration of these different compounds in the skyline?
Q3.What does these black error bar mean? Please see attach file.
Q4：Can I quantity one analyte in unknow samples with other analyte's standard curve line ? For example, I want quantity SM18:1 in unknown samples with SM18:1(d9)'s standard curve line.