|One peptide, two heavy labels?||ztflaten||2019-12-13|
Hey Skyline Forum,
I have a peptide with two labels. One is a N15 labelled full protein that is added to monitor digestion, the other has a single amino acid label. They share the same light peptide, but I've had to "duplicate" it to have both modifications present in Skyline. Is there a better way to do this? The issue ends up being if I change the integration window for one, it ends up having a different light peak area than the other.
I've attached a screenshot of what this looks like in my work space.