|mProphet scoring of removed peaks||Tobi||2019-12-11|
I wanted to report some strange behavior of Q-values and z-scores as shown in the attached screenshot and the uploaded file ,,mProphet scoring on removed peaks". (I expect it to show up pls let me know if not)
When working with DIA and peptide identification in skyline, when removing peaks manually (right-click, remove peak) and via Avant-Garde DIA, those peptides yield Q-values and Z-scores as if nothing happened. I even checked with a different scoring model and see values changing aka refreshing, but peptides where peaks were removed (see RT as well as dotp = 0) still show values from prev. existing peak boundaries. I would expect something like #N/A.
Could you please take a look at this? I guess a workaround would be to filter peptides where dotp > 0.00 or where RT fails.
Can you please also think of extending the document grid for exporting all intermediate values of the scoring? Or is there already a way to score Skyline curated data with Percolator? (When decoys might not be normally distributed etc.).
Looing forward getting in touch with you.