using external calibration curve?

support
using external calibration curve? Cedric  2019-09-09
 

Hi,

is it possible with Skyline to use the calibration curve acquired for a molecule X and apply it to another molecule Y? This will allow to do semi-quantitative analysis of molecules which do not have a specific calibration curve.

If it is not possible, do you have a way to export the calibration curve equation determined in Skyline? Like this, we could use it in excel to perform concentration calculation.

thanks for your support and your great software

Kind regards,

Cédric

 
 
Nick Shulman responded:  2019-09-15
C├ędric,

There is no way that I can think of to get Skyline to use the calibration curve from one molecule with a different molecule.

You can export the slope and intercept of the calibration curve using the Document Grid.
The columns are located under:
Proteins > Peptides > Calibration Curve (if in Proteomics Mode)
or
Molecule Lists > Molecules > Calibration Curve (in Mixed or Molecule modes)

If you want to learn more about the Document Grid you can look at the Custom Reports tutorial:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_custom_reports

-- Nick