Not able to see libray ion in the result file and how to do the quantitation of the peaks

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Not able to see libray ion in the result file and how to do the quantitation of the peaks drrenugoel  2019-08-23
 

Kindly see the attached file. I have built the library in the peptide setting tab. However, I am not able to see the library ions in the attached file (skyline_doubt). some setting has been changed as I used to get it. Kindly see the attached (skyline2).

I have run these samples on 5600 sciex in MRM-HR mode and now want to do the quantitation. Could you please let me know how to take it out peak area for the same.

 
 
Brendan MacLean responded:  2019-08-23

You should be able to switch back with View (or right-click) > Transitions > All. It appears you are currently in View > Transitions > Total mode.

Thanks for posting to the support board and supplying the screenshots.

--Brendan

 
drrenugoel responded:  2019-08-23

Thank you so much. fantastic. I had run 5 biological replicates from three conditions. I would like to do the quantitation. Could you please help?

 
Brendan MacLean responded:  2019-08-24

This is probably a good Webinar for you:

https://skyline.ms/webinar08.url

And this tutorial:

https://skyline.ms/tutorial_grouped.url