Thank you for attaching those .sky.zip files (even though the attached files have the extension ".sky", they really are zip files).
I cannot get Skyline to open the thing that is in "test-issues.sky". When I try, Skyline gives me an error about the mass of some of the product ions not being what they are calculated to be.
I see that there is an audit log in there. I can see that you inserted a transition list which looked like this:
Precursor Name Precursor m/z Precursor Charge Label Type Explicit Retention Time
Pyruvic 174 1 light 8.728
Pyruvic 175 1 heavy 8.748
Pyruvic 176 1 heavy 8.707
Pyruvic 177 1 heavy 8.769
(your transition list is much longer, but this list is sufficient to cause problems in Skyline).
With the current versions of Skyline or Skyline-Daily, what you end up with after inserting this looks like the attached image. That is, you get precursors with the m/z's 174, 175, 176, and 177, but the "transition mz" ends up being 174 for each of those things.
This is definitely a bug in Skyline. Our small molecule expert is on vacation right now. He will be able to give us a better explanation when he gets back next week.
Even though this is a bug in Skyline, I think you will be able to do what you need to do if you structure your document more the way that Skyline expects.
If you want to monitor the monoisotopic, M+1, M+2, etc ions, those should all be "transitions" under the same "precursor".
If you know the chemical formula for your molecule, you should use that instead of the number 174. When Skyline knows the chemical formula, Skyline can predict the mass of the ions in the isotope distribution.
I believe there is also some way to tell Skyline to extract chromatograms for the precursor ions in an isotope distribution even if all you have are the m/z values, but I do not know how to do that. I would have thought it would have been possible using the "Add Transition" menu item on the right-click menu for the precursors in the Targets tree. However, it looks like anything you add like that will be a custom product ion which Skyline wants to extract from MS2 scans, not MS1.
Maybe someone else on this support board will have better answers.