Peptide Transitions | iej4 | 2019-06-19 06:23 | |||||||||||||||||||||||||||||||||||
I am setting up an MRM peptide quantitation method but I need to input a non-standard transition. I have a short proline containing peptide that is producing what seems to be a strong y-18 ion but Skyline doesn't display that fragment as a possibility so I can't use it in the method. Is there a way to change the fragmentation rules of skyline so that the fragment is allowed or to manually add a transition to a peptide? Thanks. |
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