|strange behaviour of unique peptides table checkmarks||Tobi||2019-06-18|
in skyline daily 19, I used a fasta file containing several isoforms of a heatshock protein. To check for shared and unique peptides I created a proteome file from the fasta and I imported the same fasta to create the target list. When selecting all targets with CRTL+A and looking at the unique peptides table yields some unexpected results. Could you please tell me if I have an error somewhere or what might be going on?
For example Peptide EEGTQQR from protein Q8N241. In the target list and even in the table it is clearly stated that this peptides comes from this protein, in the table however there is no checkmark. Do I misunderstand the default checkmarks? Because I would expect them to occur by default in every case where a peptide matches a protein, but this is not the case. All setting were just set to default.
When clicking on a specific protein for unique peptides I havent found an error so far.
Looking forward a short reply.