Integration threshold

Integration threshold lzhangok  2019-05-22

I load 200 MRMs into Skyline and do the process, cannot find threshold parameters, so in the end, some of the metabolites have green dot indicating positive detection, where many others have not green dots indicating no detection at all. But I can confirm with the vendor Quant tool that many "not detected" metabolites can have good peaks and be detected. Hope to see your suggestions.

Brian Pratt responded:  2019-05-23
Can you provide more detail? Specifically:

Your Skyline document, using File > Share > Complete
Your raw mass spec data
A list of examples of things Skyline fails to detect but which you are convinced are there

You can upload all that to or we can arrange something else if you like.

Thanks for using the Skyline support board,

Brian Pratt
Brian Pratt responded:  2019-05-23
I see where you uploaded a file. To be clear, I'd like to see the file that is produced by Skyline's File > Share > Complete menu command (it will contain the .skyd file you did upload, but that file by itself is not useful). And of course I'd like to see the other things previously requested as well.