Try to set different collision energy for different daughter transitions of a compound

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Try to set different collision energy for different daughter transitions of a compound yufeng li18  2019-05-13
 

Hello there,

We were trying to export a method to MassLynx, which each compound has 3 to 4 daughter transitions with different collision energy. When we inserted a transition list in the Skyline, the format would look like in the picture 1 and the exported method in the MassLynx had the same collision energy (always be the collision energy for the first daughter transition in the list) for every daughter transition. We noticed the collision energy can only be modified in the precursor tab (662.1018 in the picture). In order to have correct collision energy in the MassLynx, we had to manually edited the in Skyline (shown in picture 2 ) and changed the collision energy for each precursor accordingly.

Do you have an ultimate way that can export a method from the Skyline with each daughter transitions having the correct collision in the MassLynx? I really appreciate if you could help

Many thanks
Yufeng

 
 
Brian Pratt responded:  2019-05-13

Hi Yufeng,

You are in luck! We recently addressed this issue, and collision energy is now a per-transition value instead of a precursor value. If you download the current Skyline-Daily you should find it meets your needs.

Thanks for using the Skyline support board!

Brian Pratt