Dear all,
I would like to go trough results of isobarically labeled small molecules (TMT/iTRAQ etc) that were combined for smaller number of samples and measured using scheduled PRM. Now the individual reporter ions present individual samples or standards with different concentrations and I would like to set the analyte concentration based on product m/z and replicate. For the quantification I try to set concentrations from the document grid individually, but skyline prefills all same analyte concentrations of the same replicate and same precursor regardless of different product m/z.
Is there a way to fill individual product m/z of the same precursor m/z and same replicate with different concentration for quantification with Skyline or should I just export integrated peaks and do this in a spreadsheet?
Many thanks,
Juho