Hello the awesome Skyline team!
I am doing some impurity quantification, peptides. If I have made a calibration curve from pure target protein digest with ISTD concentration of, 400 µg/ml, and injected 10µl, and I have measured unknown series, but for few technical reason I had ISTD concentration of 100 µg/ml and injection volume of 3µl, then after merging files to same skyline document, how can I get quantification's of the unknown series? When trying to apply concentration multiplier in document grid, it applies automatically to all peptides with same sequence, i.e. also to calibration curve injections. Seems not be possible to apply it to unknown samples only?