MS3 spectrum reading by skyline

MS3 spectrum reading by skyline maotian zhou  2019-04-01

Dear Skyline team,

I am trying to use skyline to read MS3 spectrum. Seems skyline can extract the MS2 spectrum through the Raw file easily. However seems there is no way to setup the MS3 level product ions, and extract the MS3 spectrum. Could you please let me know if there is a method to analysis the MS3 spectrum like what is doing on MS1 or MS2 level (can calculate area under curve).


Brian Pratt responded:  2019-04-01

Hi Maotian,

Skyline only deals with MS1 and MS2, sorry.

Thanks for using the Skyline support board,

Brian Pratt