Dear Skyline team,

sry to add to this topic but I sadly could not find this exact problem in the forum. It is more for understanding than an actual problem.

When calculating the total Area MS1 myelf in Excel and via Pivot (Sum of Areas) the number differs slightly from the Total Area MS1 from the results grid but the difference is more than what I would expect from rounding errors.

Quant is set to MS1 and DDA and Product Ions are non-quantitative (and I excluded product ion areas from the pivot sum of areas).

Is this behaviour expected or are some settings wrong? Do you have a recommendation on how to retrieve the area of a peptide as the sum of all charges and isotopes?

Thank you very much for the great support

tobi