|Fixed integration/peak boundaries||seabiscuit||2019-03-21|
Dear Skyline team,
I am doing regular SRM/MRM quantification of peptides on a Thermo TSQ Quantiva MS. We are testing a fast-and-dirty analysis process by eliminating the use of LC column for separation. So all the peptides we monitored with be introduced into the MS to form wide elution peaks that overlap with each other. The issue I have now is the algorithm Skyline uses to set the peak boundaries does not work well with this elution pattern, especially for peaks with lower intensities. So I am just wondering if you can add a feature letting the use set fixed peak boundaries for all peptides in all samples.