Hi Jason,

Could you zip that .dat file and upload to http://skyline.ms/files.url ? I'd like to investigate.

Thanks

Brian Pratt

Done, thanks
Jason

Hi Jason,
So, is S:\Users a network drive? If so, there may be some kind of issue with it and you may have better success copying your .dat file to a local disk drive. Also, if so, I doubt Brian will have much success in reproducing the issue. Failing to get the file size of the .dat file sounds pretty basic to me. Though, I expect it is the Mascot DAT reader library causing the failure, since I can't imagine why BlibBuild itself would be performing this operation.

--Brendan

Bendan-

S: is a network drive, but I've used it to make libraries from dat files many times. Also, I moved the dat file to the C: drive, tried to make a library, and get the same error (below). I think its something about this dat file specifically...

Cheers
Jason

System.IO.IOException: ERROR: Unable to read filesize of F011061_Cys_NewRat_PlusRED_0p0232.dat.
ERROR:

Command-line: C:\Users\lab_held.pcl-held1\AppData\Local\Apps\2.0\NRM38HKN.2B5\OM37C28G.N7K\skyl..tion_e4141a2a22107248_0004.0002_ed14b445d33623de\BlibBuild -s -A -H -o -c 0.99 -i test "C:\Skyline\SpectralLibraries\test.redundant.blib"
Working directory: C:\Users\Jason
Standard input:
F011061_Cys_NewRat_PlusRED_0p0232.dat

   at pwiz.Common.SystemUtil.ProcessRunner.Run(ProcessStartInfo psi, String stdin, IProgressMonitor progress, IProgressStatus& status, TextWriter writer) in C:\proj\pwiz_x64\pwiz_tools\Shared\Common\SystemUtil\ProcessRunner.cs:line 60
   at pwiz.BiblioSpec.BlibBuild.BuildLibrary(LibraryBuildAction libraryBuildAction, IProgressMonitor progressMonitor, IProgressStatus& status, String[]& ambiguous) in C:\proj\pwiz_x64\pwiz_tools\Shared\BiblioSpec\BlibBuild.cs:line 178
   at pwiz.Skyline.Model.Lib.BiblioSpecLiteBuilder.BuildLibrary(IProgressMonitor progress) in C:\proj\pwiz_x64\pwiz_tools\Skyline\Model\Lib\BiblioSpecLiteBuilder.cs:line 144

Okay, then we should be able to reproduce the problem here. Though, we may end up sending it to Matrix Science for .dat forensics. Thanks for the clarification.

Hi There,

I was just wondering if the problem with this error was found. As I am getting the same error: unable to read filesize.

Thanks,
Shannon

This error is happening because the file is more than 2GB in size.
This is a bug in BiblioSpec which should be straightforward for us to fix.

Sorry that we seem to have forgotten about this bug, and thank you for reminding us.
-- Nick

Hi Nick,

Thanks for the quick response. Is there an estimated date for when you think this might be fixed? Sorry for bugging you, I just have an experiment running in a couple of weeks I'm trying to organise.

Cheers,
Shannon

This will be fixed in the next update of Skyline-Daily, which happen every few weeks or so.

This bug was incidentally fixed (on April 23) as a part of other work that was done in terms of making sure the BiblioSpec would work with filenames that contained non-english characters.

I believe this bug has been fixed in the latest Skyline Administrator Installer if you can't wait for the next regular Skyline-Daily.

https://skyline.ms/wiki/home/software/Skyline/daily/page.view?name=install-administrator-64
--Nick

Awesome. Thanks very much :)

Hi Shannon,

As the fix is already in our codebase, you can actually have a revised BiblioSpec build right now:

https://skyline.ms/wiki/home/software/BiblioSpec/page.view?name=Download%20and%20build

Look for the "Pre-compiled binaries" section at the bottom of the page.

Best Regards,

Brian Pratt

HI

I get errors when running exec files in this bibilio spec.. Please refer to the attched screenshot..can you please send me the steps to follow once I have downloaded this folder.

Regards

It's probably just that you didn't unzip to the location where bibliospec is already installed. That download isn't a proper installer, it assumes you're updating an existing installation.

Best Regards,

Brian

If you have a normal web installation of Skyline, you can use the steps described in the "Recovering from a Broken Installation" tip to find your Skyline.exe or Skyline-daily.exe or BlibBuild.exe and put the replacement there. You probably also need to right-click on the BilbBuild.exe and BlibFilter.exe, click properties and check Unblock on this files, since Windows will block them if they were downloaded with a ZIP file and unzipped.

... Or you could just wait for the next Skyline-daily, which I will try to get out by Monday.

Great, thanks so much for your help!!