Error in standard curve

Error in standard curve mvillal1  2019-02-05

Hello Skyline team,

I am trying to plot the standard curve of 28 AQUA standards. I removed several transitions without signals from the PRM data that I collected (n=3 technical replicates per point in curve) but even for the best peptides (i.e. IALAG...) I keep getting "All of the external standards are missing one or more peaks - The selected replicate has missing or truncated transitions".

Can you please point me to something that I may be missing?

Attached is my skyline file.


Nick Shulman responded:  2019-02-05
You should go to:
Settings > Peptide Settings > Modifications
and change "Internal Standard Type" to "none".

Skyline thinks that you want to quantify your light peptides, but your Skyline document only contains heavy peptides.
-- Nick
mvillal1 responded:  2019-02-05
Hello Nick,

Thanks for the answer, is working now.