|Error in standard curve||mvillal1||2019-02-05|
Hello Skyline team,
I am trying to plot the standard curve of 28 AQUA standards. I removed several transitions without signals from the PRM data that I collected (n=3 technical replicates per point in curve) but even for the best peptides (i.e. IALAG...) I keep getting "All of the external standards are missing one or more peaks - The selected replicate has missing or truncated transitions".
Can you please point me to something that I may be missing?
Attached is my skyline file.