|Importing results of MS1-only data "raw full-scan settings must be enabled"||lparsons||2019-01-29|
I've been away from Skyline for a while (now on version 220.127.116.1109, 64bit windows; not sure what version I last used before). My goal is to take in a transition list of "parent" ions (which have not been selected for fragmentation) from MS1-only data and get AUC for every ion on the list.
I started with Edit -> Insert -> Transition List, where I pasted in a two-column list of small molecules where the first column is "Precursor m/z" and the second "Precursor Charge". In my case, all the ions in the list have a charge assumed to be +1 for now. The list imports as 1,703 molecules without error.
Then I try to import my MS1 data (Thermo raw - I've tried importing as mzML and the result is the same). File -> Import -> Results; I leave the radio button to "Add single-injection replicates in files; Optimizing "none"". I select a file and a couple seconds later I get an error from "Importing Results...":
I tried also going to Settings -> Transition Settings -> Filter -> Small Molecules. In there I set "Ion types" to "p" (for precursor) or "f,p" (full, precursor) but neither of those settings make a difference from the default of "f". Precursor adducts is at "[M+H]", fragment adducts "[M+]".
Any suggestions on this would be appreciated. I'm not sure what I need to change to resolve the "full-scan settings must be enabled" error that is preventing my files from being imported. My raw files are MS1-only acquisitions, my goal is to get AUC for the defined list of features across a number of MS1-only files. Right now I have 6 files though any one or any combination of the six will give the same error.