|CE optimize method XML parameter issue with Agilent 6495B qqq||wangqingok||2019-01-18|
Dear Skyline Group Experts,
I am using Skyline to optimize a set of over 10k peptides, and would hope to do it in a high-throughput way. I started with a batch of 200 peptides.
After I exported the method with a template method and transitions of these peptides (one method per peptide), and I put them into the worklist of the 6495B qqq, and after I initiate the run, it shows in the MassHunter Acquisition Logbook as "
However, when I just export the transitions as with CE optimizations and then copy-and-paste these transitions into the template method, it runs flawlessly in MassHunter on 6495B. I am wondering if the Skyline is actually not compatible with the parameters required by Agilent 6495B therefore the template method is not properly loaded and outputted through Skyline??? Thanks a lot!