|Lipidomics and MS1 filtering||alejandro.cohen||2019-01-17|
Hi Skyline people!
I'm setting up Skyline to analyze lipidomics samples. Running LC-MS on a QExactive, I'll be using Avanti's Splashmix of QC purposes. I've set up Skyline in MS1 Filtering mode, and successfully imported the Splashmix components and adducts. Data is so far, so good (expected adducts are there!), however, I'm not seeing the M+1 and M+2 isotope signals on each individual adduct as I typically did when I used MS1 filtering for precursors in proteomics studies. Is this correct for small molecules applications or am I doing something wrong?
Attached the .zip