Shimadzu QQQ methods with a large protein can not be predicted by skyline

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Shimadzu QQQ methods with a large protein can not be predicted by skyline spklongz  2018-12-31
 

Happy new year everyone.

Recently, I use skyline to predict LC-MS/MS method of a large protein (the fasta file of this protein is attached), while it failed to export transitions if I chose precursor charges (2,3) and ion type (y, b). If I set precursor charges (2) and ion type (y, b), it success. Do you know how to solve this problem?

Thanks.

 
 
Brendan MacLean responded:  2018-12-31

Can you show us what the error message is? Can you export a transition list (File > Export > Transition List) but just not a Shimadzu method?

 
spklongz responded:  2018-12-31

Thanks, Brendan. After I upload Skyline, it works (file > Export > Transition List).