Quick way to import hundreds of targeted proteins to Skyline?
William
2018-11-22 13:17
Hi Skyline team,
I wonder if there is a quick way to import hundreds of targeted proteins withoout creat fasta file containing all those targets?
Thanks
Brendan MacLean responded:
2018-11-22 20:35
Short answer: yes.
Long answer: depends on your starting point. What do you have? A list of accession numbers? A spectral library with a bunch of peptides?
Give us a bit more info, and I am sure we can help.
—Brendan
William responded:
2018-11-22 20:40
Thank you Brendan, I have a list of accession numbers
Brendan MacLean responded:
2018-11-22 21:17
Then you would build a background proteome of the organism FASTA and use Edit > Inserts > Proteins. If you have the backing background proteome you can just paste into the Accession column. See the Targeted Method Editing tutorial for steps to build a background proteome.
Hope this helps.
—Brendan
Brendan MacLean responded:
2018-11-23 09:56
The creation of the Yeast background proteome starts here:
The example screenshot on this page hides the Accession column, but you can just as easily paste a line-separated list of accession numbers into this column as the tutorial walks you through pasting a line-separated list to the Name column. I think you can also do this in the Preferred Name column if you had a list of those.